Fluorescence microscopy indicated that upon infection of D. discoideum producing the ER/LCV marker calnexin-GFP55,56 or the Golgi/LCV PtdIns(4)P probe P4C-GFP57–59, with L. pneumophila/Timer for 24 h, growing as well as nongrowing bacteria resided in membrane-bound compartments decorated with the GFP fusion proteins (Fig. 4c). Here, CANX is linked to infection.