To determine whether LicA inhibits osteosarcoma cell viability by inducting cell apoptosis, the U2OS and HOS cells were treated with various LicA concentrations were detected with Annexin V and dead cell assay by flow cytometry for 24 h, which resulted in a dose-dependent increase in the percentage of apoptotic cells (Figure 2A). Here, ANXA5 is linked to osteosarcoma.