qRT-PCR was used to validate the expressions of survival-associated lncRNAs and hub mRNAs resulting from WGCNA analysis, including 8 lncRNAs (CYTOR, MIR4435-2HG, RP1-137D17.2, RP11-247A12.2, RP11-646E18.4, RP11-661A12.4, RP11-661A12.5, and RP11-977B10.2) and 12 hub mRNAs (IFIT2, XAF1, UBE2L6, IFITM3, HLA-C, CTSL, ARHGDIB, LGALS3BP, IFITM1, MLKL, SERPING1, TRIM21) in cultured LC cells (Hep-2 and TU177) and one control cell (HaCaT keratinocytes) (Figure 9) [23]. The gene discussed is LGALS3BP; the disease is laryngotracheoesophageal cleft.