Upon DKK3 overexpression in 3 GBC cell lines, OCUG-1, NOZ, G-415, we observed the ability of these cell lines to form colonies was decreased significantly (p < 0.0001) (Figure 4B), suggesting DKK3 inhibits the capacity of single cells to proliferate and could act as potential tumor suppressor in GBC. This evidence concerns the gene DKK3 and neoplasm.