The cutoff value was set 478.0 ng/L (mean±1.96 SD) as the upper limit, and the incidence of serum Wnt3a was 87.8 % (158/180), which was more sensitive than that of AFP (>20 ng/mL, 67.2%, 121/180) in HCC patients (Fig.1B). Here, AFP is linked to hepatocellular carcinoma.