The up-regulated levels of Wnt3a expression in sera of HCC patients was closely associated with HBV infection (P<0.001), lymph node metastasis (P=0.016), differentiation degree (P=0.001), TNM staging (P=0.003), Child-pugh classification (P<0.001), and tumor recurrence (P=0.014). Here, WNT3A is linked to hepatocellular carcinoma.