Several studies have reported that miR-326 can inhibit cells proliferation, migration and invasion in various cancer types by targeting KRAS 20, TWIST121, LIM, SH3 protein 1 22, ELK1 23 etc. However, in our study, we showed that high expression of miR-326 is associated with worse OS in LUSC patients. The gene discussed is ELK1; the disease is cancer.