ROC curve analysis indicates that PRC1 (P < 0.001, area under curve [AUC] = 0.976, 95%CI = 0.962-0.989) and TOP2A (P < 0.001, AUC = 0.978, 95%CI = 0.965-0.991) performed well in discriminate the HBV-related HCC tumor and adjacent normal tissue. The gene discussed is TOP2A; the disease is neoplasm.