In subcutaneous tumor in nude mice, IHC showed that the Vimentin, Snail, and MMP2 positive staining were significantly more frequent in tumors of Panx1-transfected HCC cells than that in control cells, while E-cadherin protein expression was reduced in the Panx1 overexpression group, indicating that overexpression of Panx1 was more helpful to the occurrence of EMT (Figure 2D). Here, SNAI1 is linked to hepatocellular carcinoma.