In the present study, a combined strategy of the optimized native polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecylsulfonate-PAGE (SDS-PAGE) was used to separate DSHp-β chain from serum samples, and changes in the glycosylation of DSHp-β between NSCLC and BLDs were detected using matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS). The gene discussed is SH2D1A; the disease is non-small cell lung carcinoma.