In our study, EA and PB effectively improved colonic ZO-1 expression and recovered the intestinal epithelial barrier, and EA was superior to PB, which was similar to HX Shang's: after 12 weeks of acupuncture treatments on Crohn's disease, ZO-1 expression in the intestinal epithelial was increased compared to mesalazine [32]. Here, TJP1 is linked to Crohn disease.