The results of pyrophosphate sequencing (pyro-sequencing) showed that the methylation level has no significant difference in each CpG site (-31 bp, -50 bp and -168 bp) upstream of transcription starting site of MCP1 gene in CD14+ monocytes from patients with CAD versus non-CAD controls (Figures 1C, D). The gene discussed is CD14; the disease is coronary artery disorder.