Repeated LsAg administration not only improved glucose intolerance in HFD mice (Supplementary Figures 7A,B), but also significantly reduced IFN-γ+ (Figure 7B) and IL-17+ (Figure 7C) CD4+ T cell frequencies in adipose tissue compared to PBS treated HFD mice even though the body weight was comparable between the groups (data not shown). This evidence concerns the gene CD4 and glucose measurement.