Finally, we report that direct application of NTX does not affect TRPM3-dependent Ca2+ signals when tested before PregS stimulation on both IL-2 stimulated NK cells from HC and ME/CFS patients (Figures 6, 7), suggesting that NTX does not act as an agonist by directly coupling on the TRPM3 ion channel gating but may involve an upstream lasting regulatory mechanism. Here, IL2 is linked to myalgic encephalomeyelitis/chronic fatigue syndrome.