However, the soluble AβOs, extracted from 3 × Tg mice from as early as 4 months old, could bind to p100 (α3-Na/K-ATPase) in AβO ligand blot (Figure 7D), and the Na/K-ATPase activity loss was demonstrated in APP/PS1 mice brains, which suggests that oligomers of Aβ may inhibit activity of Na/K-ATPase in early stages of AD. This evidence concerns the gene ABO and Alzheimer disease.