We then sought to examine the effects of acute CNO (0.5 mg/kg)-mediated hM4Di DREADD inhibition of CamKIIα-positive forebrain excitatory neurons on anxiety-like behaviour in the OFT, EPM, and LD avoidance test and on despair-like behaviour in the TST and FST paradigms (Figure 5A). This evidence concerns the gene CAMK2A and Anxiety.