For example, SRGN that encodes a hematopoietic cell granule proteoglycan, has been identified by CES as a cell-specific essential gene for eight leukaemia cell lines, of which seven were not detected by CRISPR or shRNA-screens alone, including MOLM13, MV411, MONOMAC1, OCIAML2, OCIAML3, OCIAML5, and THP1. The gene discussed is GLI2; the disease is leukemia.