More importantly, our animal studies revealed that aspirin abolished the 5-Fu-induced up-regulated expression of p-p65 and p-IKK-β Consistent with our Western blotting results, we discovered that aspirin strongly suppressed the DNA binding activity of NF-κB and attenuated the 5-Fu-induced NF-κB activation in tumor samples (Fig. 5B). Here, NFKB1 is linked to neoplasm.