This study was the first to demonstrate that aspirin effectively re-sensitized the chemoresistant CRC cells to 5-Fu and potentiated the antitumor effects of 5-Fu on chemoresistant CRC cells without inducing additional AEs, which was achieved through suppressing the 5-Fu-induced activation of the NF-κB pathway. Here, NFKB1 is linked to colorectal carcinoma.