Significantly, AMPK blockage failed to completely reverse Lnc-THOR shRNA-induced cytotoxicity in A172 glioma cells, suggesting that both AMPK-dependent and AMPK-independent mechanisms are responsible for Lnc-THOR-silencing-induced glioma cell death (see Fig. 8, the proposed signaling pathway of the study). The gene discussed is PRKAA1; the disease is central nervous system cancer.