We chose the human immortalized liver cell line (HL-7702) and several HCC cell lines (Huh7, Hep3B, SMMC-7721, HepG2, and SK-Hep1) for this analysis, and found that NSD1 had the highest expression in SK-Hep1 cell line and the lowest expression in HepG2 cell line, suggesting that expression of NSD1 in HCC cell lines was significantly higher than that in the normal cell line (Fig. 2a). This evidence concerns the gene NSD1 and hepatocellular carcinoma.