Knockout of NSD1 mediated by CRISPR/Cas9 system promotes H3K27me3 methylation and inhibits Wnt10b transcription, thereby suppressing activation of the Wnt/β-catenin signaling pathway (Fig. 6), which finally impedes cell proliferation, migration and invasion in HCC. This evidence concerns the gene WNT10B and hepatocellular carcinoma.