We observed that compared with control cells, the degradation rates of ectopically expressed PD-L1 did not increase in EZH2-silenced hepatoma cells after treatment with the protein synthesis inhibitor cycloheximide (CHX), which suggested that the regulation by EZH2 of IFNγ-induced PD-L1 does not involve the protein stability of PD-L1 in hepatoma cells (Additional file 1: Figure S8). This evidence concerns the gene IFNG and hepatocellular carcinoma.