AKT1 and glioma: To characterize the 5-methylcystosine (5mC) methylation level in cells at the origin of the Akt + diuron-induced glioma (Ntv-a/Akt + diuron cells), we next compared the methylation level of DNA extracted from Ntv-a/LacZ (control, Ctrl), Ntv-a/Ras + Akt, and Ntv-a/Ras + Akt + folate cells.