Furthermore, we investigated the changes in the KC and MIP‐2 contents in the TPA‐induced irritant dermatitis because mouse KC and MIP‐2 are reportedly the functional analogues of human IL‐8 (a neutrophil chemotactic factor).20, 21, 22 As shown in Figure 2B and 2C, the TPA application resulted in an increase in the KC and MIP‐2 protein levels in the ear skin and LCZ significantly reduced both the KC and MIP‐2 levels in a dose‐dependent manner. This evidence concerns the gene CALCA and irritant dermatitis.