In contrast, Himmel et al. (34) revealedthat Helios+ and Helios- nTregs are not different in theirfunctional properties for suppressing T cell proliferation.In the present study, we investigated the expression ofHelios in the population of both CD4+CD25+FOXP3+and CD4+CD25-FOXP3+ Tregs and the results revealedexpansion of this subset in both population after exposingthe cells to ASCs, specially to cancer ASCs. The gene discussed is CD4; the disease is cancer.