In this large Chinese cohort study, we evaluated HER2 status in CRC based on two different criteria and found that HER2 positivity in colorectal adenocarcinoma was quite low in Chinese population, with a rate of 2.9% according to the HER2 diagnostic criteria for gastroesophageal adenocarcinoma, and a rate of 2.6% according to the HERACLES criteria. This evidence concerns the gene ERBB2 and colorectal adenocarcinoma.