ERBB2 and colorectal carcinoma: Valtorta E et al. [24] developed the HERACLES diagnostic criteria for HER2 in CRC: tumors with a 3+ HER2 score in more than 50% of cells by IHC or tumors with a 2+ HER2 score and a HER2:CEP17 ratio higher than two in more than 50% of cells by FISH.