In the discordant group analysis comparing gene expression in ASC affected compared to unaffected co-twins, three DE genes were identified at FDR < 10%: IGHG4, EVI2A and SNORD15B. The case control analysis examining expression differences between ASC affected and control samples identified four DE genes at FDR < 10% including IGHG4, DEPDC1B and ZNF501. Comparison of mean within-pair logFC expression differences revealed that differences were greater in the discordant ASC pairs than in concordant ASC and control pairs. The gene discussed is ZNF501; the disease is Tako-tsubo cardiomyopathy.