The present experiment showed the contents of collagen-1, collagen-2, and TGF-β did not change significantly with no intervention, and there was no significant degeneration of the NP and AF in the control group, while under the conditions of +Gz and/or microgravity, the contents of collagen-1 and TGF-β gradually increased and that of collagen-2 decreased with an increase in exposure time. Here, TGFB1 is linked to atrial fibrillation.