In this study, we designed a series of functional and molecular assays to explore the ceRNA mechanism of circGRAMD1B and found that phosphatase and tensin homolog (PTEN) and cyclin dependent kinase inhibitor 1A (CDKN1A (p21, Cip1)) constitute a ceRNA regulatory network for the circGRAMD1B/miR-130a-3p axis in GC. Here, CDKN1A is linked to gastric cancer.