In this study, we revealed that DAPT treatment not only markedly reduced the injury-induced myofibroblastic phenotype and fibrosis via inhibiting the EMT and FMD but also exerted its inhibitory effect on TGF-β1-mediated fibrotic changes, identifying a crosstalk between Notch1 and TGF-β1 in renal fibrosis. The gene discussed is NOTCH1; the disease is renal fibrosis.