Although there was a trend towards greater prevalence of mechanic’s hands, meeting diagnostic criteria for CTD, and higher anti-Ro52 titer in patients with an additional myositis-specific antibody, after correcting for multiple comparisons only a positive cytoplasmic antibody was statistically significantly associated with the presence of an additional positive myositis-specific antibody compared to anti-Ro52 alone (p = 0.002). The gene discussed is TRIM21; the disease is myositis disease.