It has been demonstrated that autophagy may be helpful to protect liver from ethanol-induced hepatotoxicity,24 nonalcoholic fatty liver disease,25 and CCl4-induced liver fibrosis.26 According to the animal experiment, we found that the PU groups had significantly higher expressions of Beclin1 than the CCl4 group. Here, BECN1 is linked to metabolic dysfunction-associated steatotic liver disease.