In mice fed with MCD diet to induce NASH, selective hepatocyte deficiency of TLR5 was shown to exacerbate liver inflammation and fibrosis via elevated expression of TNF-α, monocyte chemoattractant protein (MCP)1, and IL-1β, as well as Timp1, Mmp9, Col1, and collagen deposition [125]. The gene discussed is TLR5; the disease is metabolic dysfunction-associated steatohepatitis.