Animals only treated with the peptide vaccine (AWE) showed reduced tumor-growth rates compared to controls (p < 0.01 compared to PBS); this reduction was more evident in mice treated with two cycles of the combination of granulocyte-macrophage colony-stimulating factor (GM-CSF)/AWE (p < 0.0001 compared to AWE), while the most significant delay in melanoma tumor growth was recorded in mice therapeutically treated with two cycles of the combined proTα/AWE or proTα(100–109)/AWE preparations. The gene discussed is CSF2; the disease is melanoma.