ANXA5 and esophageal squamous cell carcinoma: Early apoptosis percentage of Annexin V+/7-AAD- gating was increased to 9.69 ± 0.17% or 16.79 ± 1.12%, while the late apoptosis percentage of Annexin V+/7-AAD+ gating was increased to 27.68 ± 1.53 or 19.02 ± 0.83% in KYSE 30 or KYSE 450 ESCC cells after treatment with 15 μM Ech, respectively (Figure 2c).