AIF1 and hydrops fetalis: The HF feeding led to a significant increase in IBA1-positive staining intensity in the NTS compared to HFRS (HF 0.065 ± 0.024 versus HFRS 0.011 ± 0.003, p < 0.05), but this did not reach significance when compared to chow controls (chow 0.025 ± 0.007 versus HF, p = 0.127) (Figure 6).