Tim-3 expression on splenic CD8+ T cells (Fig. 1a, e, ANOVA: F(5, 24)= 243.822, P < 0.0001), CD49b+ cells (Fig. 1b, f, ANOVA: F(5, 24)= 8.833, P < 0.0001), and CD4+ T cells (Fig. 1c, g, ANOVA: F(5, 24)= 33.384, P < 0.0001) gradually increased during the infection periods. This evidence concerns the gene CD4 and infection.