To determine whether there is single cell variation in HIV infection or transcription within cell lines, we measured levels of HIV TAR, LongLTR, Gag, Pol, Nef, PolyA and Tat-Rev at the single cell level and visualized the range in levels across all cells using violin plots (Fig. 3a) and heat maps (Fig. 3b). The gene discussed is S100B; the disease is HIV infectious disease.