When T24 cells with overexpression or silencing of TFCP2L1 were seeded on low attachment plates at clonogenic densities, followed by a 1‐week culture period, we found that TFCP2L1‐silenced T24 BC cells hardly produced tumor spheres with sharp edges; however, ectopic expression of TFCP2L1 or its co‐expression with CDK1 increased tumor sphere formation in comparison with cells transfected with the empty control (Fig 5F). This evidence concerns the gene TFCP2L1 and breast cancer.