Combined with the results of the dual-luciferase assay showing that miR-622 could bind to RNF8 at its 3'-UTR to induce posttranscriptional silencing, we conclude that miR-622 can directly regulate RNF8 in breast cancer cells, verifying the miR-622-RNF8 axis. The gene discussed is RNF8; the disease is breast carcinoma.