RNF8 and breast cancer: To further test whether the miR-622-induced EMT process and EMT-related phenotype changes (for example, migration) of breast cancer cells are directly dependent on RNF8, an siRNA designed against RNF8 (siRNF8) and a miR-622-inhibitor (antagomir) were cotransfected to knock down the expression of RNF8 and miR-622 in breast cancer cell MCF7, respectively (Figure 9A).