CCN1 and breast cancer: Furthermore, increased extracellular CYR61 expression increases 3D spheroid invaded area of non-invasive estrogen positive breast cancer cells (Figure 1E; MCF-7 rhCYR61: 119.7 ± 2.93 SEM invaded area in % to MCF-7 control; n = 5; P = 0.001; T47D rhCYR61: 128.6 ± 4.38 SEM invaded area in % to T47D control; n = 4; P = 0.0006).