Based on the pathological diagnosis, in 54 cervical tumor tissues, the Tim-3 high-frequency subgroup had a higher percentage (52%, 11/21) of poorly differentiated cancer types than had the Tim-3 low-frequency subgroup (27%, 9/33) in cervical cancer patients, although we did not detect statistical significance of Tim-3+ CD8 TIL frequency between poorly differentiated and moderately differentiated cervical cancer patients (Figure 5B, Supplementary Figure 3A). This evidence concerns the gene CD8A and cervical cancer.