Control assays showed that ascitic fluid T cell activation (detected as increase in CD107a+/CD137+ cells in the lymphocyte-tumor-scDb co-cultures) could be induced in CD8+ T cells only by the TRAIL-R2xCD3 scDb, but not by the Mec14xUCHT1 scDb (Supplementary Figure 7). Here, TNFRSF9 is linked to neoplasm.