TNFRSF10B and melanoma: Moreover, we demonstrated that co-culture of non-activated PBLs with TRAIL-R2+ melanoma cells in the presence of the scDb also led to activation of CD4+ and CD8+ T-cell proliferation, as shown by CFSE assay (Figures 5A,B), but not of CD3−/CD19+ cells (Supplementary Figure 4).