TNF and hypertensive disorder: To test the effect of 8 weeks of aerobic ExT or PVN infusion of TLR4 inhibitor in hypertension, we respectively examined the PVN levels of TNF-α and IL-1β mRNA (Figures 7C,D) and protein (Figures 7A,B, 8, 9) by RT-qPCR, western blotting, and immunofluorescence staining.