To further study the function of CKS1 in colon cancer, lentiviral-coated CKS1 was transfected into HCT116 cells, and this CKS1-overexpressing HCT116 cells were injected into 6-week-old BALB/c nude mice, whereas GFP-overexpressing HCT116 cells was used to inject 6-week-old BALB/c nude mice as the control group. Here, CKS1B is linked to malignant colon neoplasm.