Additionally, the expression level of EMT (epithelial-mesenchymal transition) landmark proteins including E-cadherin, Vimentin, Cytokeratin and MMP-9 were also assessed by immunofluorescence, results showed that ENMD-2076 inhibit glioblastoma migration and invasion ability by suppressing EMT process. The gene discussed is CDH1; the disease is glioblastoma.