The diagnostic and prognostic role of urinary NGAL has been the focus of recent preliminary studies in dogs.20, 21, 22, 23, 24 The concentration of NGAL seemed able to predict azotemic AKI earlier than did sCr, to correctly detect non‐azotemic AKI and to predict renal recovery.21, 22, 23, 24 Increased uNGAL concentrations have been reported during both ischemic and toxic AKI.20, 25 However, nonrenal sources of NGAL have been detected in dogs, as in humans, challenging its specificity for AKI especially during inflammatory states.26 This evidence concerns the gene LCN2 and acute kidney injury.