CHAT and amyotrophic lateral sclerosis: When examined for human TDP-43 expression in spinal motor neurons, all ChAT-tTA/TRE-TDP-43M337V rats demonstrated TDP-43 pathology consistent with that observed in human ALS including nuclear cytoplasmic inclusions (Fig. 3b), nuclear depletion of TDP-43 (Fig. 3c), and punctate nuclear TDP-43 deposition (Fig. 3d).