To assess the level of AQP9 protein expression in FUSCC tumor samples, we performed IHC staining and found significant elevated AQP9 expression in terms of density and intensity in ccRCC tissues compared with adjacent normal kidney tissues in FUSCC cohort (p < 0.001, Fig. 3c, d). The gene discussed is AQP9; the disease is nonpapillary renal cell carcinoma.