Here, we showed that the LSK compartment was significantly enlarged in DKI AML mice compared with the diseased Dnmt3aR878H/+ mice, suggesting that DNMT3A mutation with acquisition of additional genetic abnormality such as NRAS mutation could significantly promote the leukemogenic transformation and proliferation of hematopoietic cells. The gene discussed is DNMT3A; the disease is acute myeloid leukemia.