NFKB1 and hepatocellular carcinoma: Then, those parental and radioresistant HCC cells were transfected with a NF-κB-dependent luciferase reporter plasmid (2 × NF-κB-Luc), and assays of luciferase activity in lysates indicated that the NF-κB activity was significantly increased in HepG2-R cells in comparison with their parental cells (Fig. 6c).