CCND1 and acute myeloid leukemia: Treatment of AML cell lines with 5-aza-2′-deoxycytidine, a demethylating agent, and the resulting re-expression of Wnt antagonists led to an inactivation of the Wnt pathway as detected through the Wnt pathway genes (e.g., cyclin D1), TCF1 and LEF1 downregulation and by the reduction of nuclear localization of β-catenin [31].